Mairine Organism DNA essays

Mairine Organism DNA essays The Ocean Genome Legacy (OGL) is a not-for-profit organization set up in 2001 by New England Biolabs. The goals of OGL are to assemble a team of scientists dedicated to: The long-term preservation of marine organism DNA The development of tissue culture techniques for marine invertebrate cells The construction of genome libraries and their distribution for research purposes Studies of evolutionary genomics, with an initial focus on marine transition organisms Much of the worlds biodiversity is in the oceans and provides an important resource for mankind. Equally much of this diversity is endangered and OGL aims to help in the global effort to preserve and study the organisms of the ocean. Initially, OGL will focus on endangered marine organisms especially those that represent important evolutionary transitions. Oversight for OGL is provided by a Board of Trustees including Dr. John Benzie, Dr. Sydney Brenner, Dr. Donald Comb, Mr. Douglas Foy, Dr. Richard Roberts and Dr. Wolfgang Sterrer. Isolated from a recombinant source Description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a non-processive 5 -> 3 polymerase activity and a double-strand specific 5 -> 3 exonuclease activity. Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YTI. Reaction Buffer: 1X ThermoPol Reaction Buffer 10 mM KCl, 10 mM (NH4)2S04, 20 mM Tris-HCl, 2 mM MgS04, 0.1% Triton X-100, pH 8.8 @ 25C. Quality Assurance: Purified free of contaminating endonucleases and exonucleases. Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75C. Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 Â µM each dNTP including [3H]-dTTP and 200 Â µg/ml ...